Aperçu: G.M.
L'infection
virale prénatale a été identifiée comme un facteur de risque potentiel
pour le développement de troubles neurodéveloppementaux tels que la
schizophrénie et l'autisme.
L'étude a caractérisé les profils de développement de marqueurs
sélectionnés pour ces systèmes dans des cérébelles de souris nées de
souris enceintes infectées par le virus de la grippe humaine (H1N1) .
Le
cervelet a été choisi du fait des preuves émergentes selon lesquelles il joue un rôle dans l'apprentissage, la mémoire et le traitement émotionnel,
tous perturbés dans l'autisme et la schizophrénie.
L'étude fournit des preuves de perturbation de la FMRP, de la glutamatérgie et de la
signalisation de Reelin chez la progéniture de souris exposée qui
explique les multiples anomalies cérébrales observées dans ce modèle
animal.
J Neurosci Res. 2017 May;95(5):1110-1122. doi: 10.1002/jnr.23949. Epub 2016 Oct 13.
The effects of prenatal H1N1 infection at E16 on FMRP, glutamate, GABA, and reelin signaling systems in developing murine cerebellum
Fatemi SH1,2, Folsom TD1, Liesch SB1, Kneeland RE1, Karkhane Yousefi M1,3, Thuras PD4.
Author information
- 1
- Department of Psychiatry, Division of Neuroscience Research, University of Minnesota Medical School, Minneapolis, Minnesota.
- 2
- Department of Neuroscience, University of Minnesota Medical School, Minneapolis, Minnesota.
- 3
- Department of Psychiatry and Behavioral Neurosciences, Wayne State University, Detroit, Michigan.
- 4
- VA Medical Center, Department of Psychiatry, Minneapolis, Minnesota.
Abstract
Prenatal
viral infection has been identified as a potential risk factor for the
development of neurodevelopmental disorders such as schizophrenia and autism.
Additionally, dysfunction in gamma-aminobutyric acid, Reelin, and
fragile X mental retardation protein (FMRP)-metabotropic glutamate
receptor 5 signaling systems has also been demonstrated in these two
disorders. In the current report, we have characterized the
developmental profiles of selected markers for these systems in
cerebella of mice born to pregnant mice infected with human influenza
(H1N1) virus on embryonic day 16 or sham-infected controls using
SDS-PAGE and Western blotting techniques and evaluated the presence of
abnormalities in the above-mentioned markers during brain development.
The cerebellum was selected in light of emerging evidence that it plays
roles in learning, memory, and emotional processing-all of which are
disrupted in autism
and schizophrenia. We identified unique patterns of gene and protein
expression at birth (postnatal day 0 [P0]), childhood (P14), adolescence
(P35), and young adulthood (P56) in both exposed and control mouse
progeny. We also identified significant differences in protein
expression for FMRP, very-low-density lipoprotein receptor, and glutamic
acid decarboxylase 65 and 67 kDa proteins at specific postnatal time
points in cerebella of the offspring of exposed mice. Our results
provide evidence of disrupted FMRP, glutamatergic, and Reelin signaling
in the exposed mouse offspring that explains the multiple brain
abnormalities observed in this animal model. © 2016 Wiley Periodicals,
Inc.
© 2016 Wiley Periodicals, Inc.
- PMID: 27735078
- PMCID: PMC5352480 [Available on 2017-11-01]
- DOI: 10.1002/jnr.23949
