Aperçu: G.M.
Nous avons généré et caractérisé sept lignées de cellules souches pluripotentes induites par l'homme (iPSC) dérivées de cellules mononucléées du sang périphérique (PBMC) appartenant à une seule famille, y compris des personnes non atteintes et affectées cliniquement diagnostiquées avec un "trouble du spectre de l'autisme" (dTSA). La reprogrammation des PBMC a été réalisée à l'aide du virus Sendai non intégratif contenant les facteurs de reprogrammation POU5F1 (OCT4), SOX2, KLF4 et MYC.
Toutes les lignées iPSC présentaient un caryotype normal et la pluripotence était validée par immunofluorescence, cytométrie en flux et leur capacité à se différencier dans les trois couches germinales embryonnaires.
Ces lignées iPSC constituent une ressource précieuse pour l’étude des mécanismes moléculaires sous-jacents des TSA.
Stem Cell Res. 2019 Aug 1;39:101516. doi: 10.1016/j.scr.2019.101516.
Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder
Bozaoglu K1, Gao Y1, Stanley E2, Fanjul-Fernández M3, Brown NJ4, Pope K5, Green CC6, Vlahos K7, Sourris K7, Bahlo M8, Delatycki M3, Scheffer I9, Lockhart PJ10.
Author information
- 1
- Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia.
- 2
- Department of Paediatrics, University of Melbourne, Parkville, Australia; Murdoch Children's Research Institute, Parkville, Australia; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australia.
- 3
- Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia; Victorian Clinical Genetics Services, Victoria, Australia.
- 4
- Victorian Clinical Genetics Services, Victoria, Australia.
- 5
- Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia.
- 6
- Olga Tennison Autism Research Centre, School of Psychology and Public Health, La Trobe University, Bundoora, Victoria, Australia; Department of Medicine, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australia.
- 7
- Murdoch Children's Research Institute, Parkville, Australia.
- 8
- The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology, University of Melbourne, Melbourne, Australia.
- 9
- Department of Paediatrics, University of Melbourne, Parkville, Australia; Department of Medicine, University of Melbourne, Austin Health, Melbourne, VIC, Australia; Florey Institute, Melbourne, VIC, Australia; Department of Neurology, Royal Children's Hospital, Melbourne, Australia.
- 10
- Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia. Electronic address: paul.lockhart@mcri.edu.au
Abstract
We
have generated and characterized seven human induced pluripotent stem
cell (iPSC) lines derived from peripheral blood mononuclear cells
(PBMCs) from a single family, including unaffected and affected
individuals clinically diagnosed with Autism Spectrum Disorder
(ASD). The reprogramming of the PBMCs was performed using
non-integrative Sendai virus containing the reprogramming factors POU5F1
(OCT4), SOX2, KLF4 and MYC. All iPSC lines exhibited a normal karyotype
and pluripotency was validated by immunofluorescence, flow cytometry
and their ability to differentiate into the three embryonic germ layers.
These iPSC lines are a valuable resource to study the molecular
mechanisms underlying ASD.
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
- PMID:31415975
- DOI:10.1016/j.scr.2019.101516
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