L'excitotoxicité du glutamate induite par l'acide propionique administré par voie orale, un acide gras à chaîne courte peut être améliorée par le pollen d'abeille

Aperçu: G.M.

Les modèles de rongeurs peuvent guider les recherches visant à identifier soit des neuro-toxiques environnementaux, soit des médicaments ayant des effets neuro-thérapeutiques.Ce travail vise à étudier les effets thérapeutiques du pollen d'abeille sur l'excitotoxicité du glutamate cérébral et le l'altération du circuit glutamine-glutamate-gamma amy-butyrique (GABA) induit par l'acide propionique (PPA), un acide gras à chaîne courte, chez les petits de rat. 

Les résultats ont montré que le PPA a provoqué de multiples signes d'excitotoxicité, mesurés par l'élévation du glutamate et du ratio glutamate / glutamine et la diminution de GABA, de la glutamine et du ratio GABA / glutamate. Le pollen d'abeille a été efficace pour contrer les effets neurotoxiques de la PPA dans une certaine mesure.En conclusion, le pollen d'abeille démontre des effets d'amélioration sur l'excitotoxicité du glutamate et sur l'altération du circuit glutamine-glutamate-GABA comme deux mécanismes étiologiques dans la neurotoxicité induite par le PPA.

Lipids Health Dis. 2017 May 22;16(1):96. doi: 10.1186/s12944-017-0485-7.

Glutamate excitotoxicity induced by orally administered propionic acid, a short chain fatty acid can be ameliorated by bee pollen

El-Ansary A^1,2,3,4, Al-Salem HS⁵, Asma A⁶, Al-Dbass A⁷.

Author information

1

Central Laboratory, Female Center for Medical Studies and Scientific Section, King Saud University, Riyadh, Saudi Arabia. afafkelansary@gmail.com.

2

Autism Research and Treatment Center, Riyadh, Saudi Arabia. afafkelansary@gmail.com.

3

Shaik AL-Amodi Autism Research Chair, King Saud University, Riyadh, Saudi Arabia. afafkelansary@gmail.com.

4

Medicinal Chemistry Department, National Research Centre, Dokki, Cairo, Egypt. afafkelansary@gmail.com.

5

Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.

6

Central Laboratory, Female Center for Medical Studies and Scientific Section, King Saud University, Riyadh, Saudi Arabia.

7

Department of Biochemistry, Science College, King Saud University, Riyadh, Saudi Arabia.

Abstract

BACKGROUND:

Rodent models may guide investigations towards identifying either environmental neuro-toxicants or drugs with neuro-therapeutic effects. This work aims to study the therapeutic effects of bee pollen on brain glutamate excitotoxicity and the impaired glutamine-glutamate- gamma amino butyric acid (GABA) circuit induced by propionic acid (PPA), a short chain fatty acid, in rat pups.

METHODS:

Twenty-four young male Western Albino rats 3-4 weeks of age, and 45-60 g body weight were enrolled in the present study. They were grouped into four equal groups: Group 1, the control received phosphate buffered saline at the same time of PPA adminstration; Group 2, received 750 mg/kg body weight divided into 3 equal daily doses and served as acute neurotoxic dose of PPA; Group 3, received 750 mg/kg body weight divided in 10 equal doses of 75 mg/kg body weight/day, and served as the sub-acute group; and Group 4, the therapeutic group, was treated with bee pollen (50 mg/kg body weight) for 30 days after acute PPA intoxication. GABA, glutamate and glutamine were measured in the brain homogenates of the four groups.

RESULTS:

The results showed that PPA caused multiple signs of excitotoxicity, as measured by the elevation of glutamate and the glutamate/glutamine ratio and the decrease of GABA, glutamine and the GABA/glutamate ratio. Bee pollen was effective in counteracting the neurotoxic effects of PPA to a certain extent.

CONCLUSION:

In conclusion, bee pollen demonstrates ameliorating effects on glutamate excitotoxicity and the impaired glutamine-glutamate-GABA circuit as two etiological mechanisms in PPA-induced neurotoxicity.

PMID: 28532421

DOI: 10.1186/s12944-017-0485-7