Sérum glycopatternique et glycoprotéines de liaison à la lectine-II de Maackia amurensis dans le trouble du spectre de l'autisme

Aperçu: G.M.

On sait peu de choses sur les altérations de la glycosylation et des glycoprotéines dans les TSA.

Dans cette étude, les sérum des modèles de glucoside et les glycoprotéines de liaison à la lectine-II de maackia amurensis (MBG) chez 65 enfants avec un diagnostic de TSA et 65 enfants au  développement typique (TD) appariés selon l'âge ont été comparés.

L'analyse bioinformatique a révélé une cascade de complément anormale et une régulation aberrante de la réponse à la stimulation qui pourrait être de nouveaux fabricants ou marqueurs pour les TSA. L'altération de l'expression de maackia amurensis (MBG) et leur sialoglycosylation (?) peuvent servir de biomarqueurs potentiels pour le diagnostic de TSA et fournir des informations utiles pour les recherches sur la pathogenèse des TSA.

Sci Rep. 2017 May 9;7:46041. doi: 10.1038/srep46041.

Serum glycopattern and Maackia amurensis lectin-II binding glycoproteins in autism spectrum disorder

Qin Y¹, Chen Y², Yang J¹, Wu F¹, Zhao L¹, Yang F³, Xue P³, Shi Z⁴, Song T¹, Huang C¹.

Author information

1

Department of Cell Biology and Genetics, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, P. R. China.

2

Xi'an Child's Hospital of Medical College of Xi'an Jiaotong University, Xi'an Child's Hospital, Xi'an 710002, P. R. China.

3

Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, P. R. China.

4

The Department of Biology, College of Liberal Arts and Science, The University of Iowa, Iowa 430015, USA.

Abstract

The pathophysiology of autistic spectrum disorder (ASD) is not fully understood and there are no diagnostic or predictive biomarkers. Glycosylation modified as many as 70% of all human proteins can sensitively reflect various pathological changes. However, little is known about the alterations of glycosylation and glycoproteins in ASD. In this study, serum glycopattern and the maackia amurensis lectin-II binding glycoproteins (MBGs) in 65 children with ASD and 65 age-matched typically developing (TD) children were compared by using lectin microarrays and lectin-magnetic particle conjugate-assisted LC-MS/MS analyses. Expression of Siaα2-3 Gal/GalNAc was significantly increased in pooled (fold change = 3.33, p < 0.001) and individual (p = 0.009) serum samples from ASD versus TD children. A total of 194 and 217 MGBs were identified from TD and ASD sera respectively, of which 74 proteins were specially identified or up-regulated in ASD. Bioinformatic analysis revealed abnormal complement cascade and aberrant regulation of response-to-stimulus that might be novel makers or markers for ASD. Moreover, increase of APOD α2-3 sialoglycosylation could sensitively and specifically distinguish ASD samples from TD samples (AUC is 0.88). In conclusion, alteration of MBGs expression and their sialoglycosylation may serve as potential biomarkers for diagnosis of ASD, and provide useful information for investigations into the pathogenesis of ASD.

PMID:28485374

PMCID: PMC5423032

DOI:10.1038/srep46041