Aperçu: G.M.
En plus de la fonction motrice, le cervelet a été impliqué dans les comportements cognitifs et sociaux. Diverses
anomalies structurelles et fonctionnelles des cellules de Purkinje (PC)
ont été observées dans la schizophrénie et l'autisme.
Les résultats
indiquent que le DISC1 mutant modifie la physiologie des PC, ce qui
peut conduire à une mémoire de reconnaissance anormale chez la souris.
Neurobiol Dis. 2017 Apr 6. pii: S0969-9961(17)30082-7. doi: 10.1016/j.nbd.2017.04.008.
Expression of mutant DISC1 in Purkinje cells increases their spontaneous activity and impairs cognitive and social behaviors in mice
Shevelkin AV1, Terrillion CE2, Abazyan BN2, Kajstura TJ3, Jouroukhin YA2, Rudow GL4, Troncoso JC4, Linden DJ3, Pletnikov MV5.
Author information
- 1
- Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA; P.K. Anokhin Research Institute of Normal Physiology, Moscow, Russian Federation.
- 2
- Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
- 3
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
- 4
- Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
- 5
- Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: mpletnik@jhmi.edu
Abstract
In
addition to motor function, the cerebellum has been implicated in
cognitive and social behaviors. Various structural and functional
abnormalities of Purkinje cells (PCs) have been observed in
schizophrenia and autism.
As PCs express the gene Disrupted-In-Schizophrenia-1 (DISC1), and DISC1
variants have been associated with neurodevelopmental disorders, we
evaluated the role of DISC1 in cerebellar physiology and associated
behaviors using a mouse model of inducible and selective expression of a
dominant-negative, C-terminus truncated human DISC1 (mutant DISC1) in
PCs. Mutant DISC1 male mice demonstrated impaired social and novel
placement recognition. No group differences were found in
novelty-induced hyperactivity, elevated plus maze test, spontaneous
alternation, spatial recognition in Y maze, sociability or accelerated
rotarod. Expression of mutant DISC1 was associated with a decreased
number of large somata PCs (volume: 3000-5000μm3) and an increased number of smaller somata PCs (volume: 750-1000μm3)
without affecting the total number of PCs or the volume of the
cerebellum. Compared to control mice, attached loose patch recordings of
PCs in mutant DISC1 mice revealed increased spontaneous firing of PCs;
and whole cell recordings showed increased amplitude and frequency of
mEPSCs without significant changes in either Rinput or
parallel fiber EPSC paired-pulse ratio. Our findings indicate that
mutant DISC1 alters the physiology of PCs, possibly leading to abnormal
recognition memory in mice.
Copyright © 2017. Published by Elsevier Inc.
- PMID: 28392471
- DOI: 10.1016/j.nbd.2017.04.008
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